• mei 1, 2021
sexpert-vlaanderen

Antimicrobiële activiteit van kamferboomzilvernanodeeltjes

Antimicrobiële activiteit van kamferboomzilvernanodeeltjes tegen vuilbroedziekten en het ontdekken van een nieuwe stam van Serratia marcescens through DGGE-PCR als secundaire infectie op honingbijlarven

American foulbrood (AFB) and European foulbrood (EFB) are the 2 main bacterial illnesses affecting honeybees, resulting in a lower in viability of the hive, reducing honey manufacturing, and leading to important financial losses to beekeepers. As a result of inefficiency and/or low efficacy of some antibiotics, researches with nanotechnology symbolize, probably, new therapeutic methods. Nanostructure medicine have offered some advantagesover the standard medicines, resembling gradual, gradual and managed launch, elevated bioavailability, and diminished side-effects.

On this research, completely different contaminated larvae had been collected from two apiaries; the combs that had signs of American and European foulbrood had been remoted. In vitro antimicrobial exercise of camphor tree silver nano-particles towards foulbrood illnesses had been characterised utilizing UV-Vis spectrophotometry and scanning electron microscope (SEM) that proves the formation of silver nanoparticles with measurement vary 160-660 nm. The antimicrobial exercise of the silver nanoparticles was examined utilizing agar diffusion assay and proved their potential to successfully stop the pathogenic bacterial development in each AFB and EFB.

DGGE-PCR approach has been utilized for the identification of un-common bacterial infections honeybees relying on 16S rRNA amplification from their complete extracted DNA and has been recognized as Serratia marcescens (TES), deposited in GenBank with a brand new accession quantity (MT240613). The outcomes had been confirmed pressure has been detected by DGGE-PCR evaluation inflicting uniquely contaminated brood that was attacked by the American Foulbrood It could possibly be concluded that greenly synthesized silver nanoparticles is projected for use as efficient remedy for honeybee bacterial illnesses. These materials want extra investigations underneath area circumstances and research the risk of its residues in honeybee merchandise resembling honey, and beeswax.

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Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn)

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Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn)

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Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)

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Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn)

31015-1 5x1mL
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Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn)

31015-2 50x1mL
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31042-1 5x1mL
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions)

31042-20mL 2x10mL
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions)

31042-T 1mL
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Taqman Master Mix-ROX

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amfiSure qGreen Q-PCR Master Mix(2X), No Rox

Q5600-001 1ml
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amfiSure qGreen Q-PCR Master Mix(2X), No Rox

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amfiSure qGreen Q-PCR Master Mix(2X), No Rox

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amfiSure qGreen Q-PCR Master Mix(2X), High Rox

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amfiSure qGreen Q-PCR Master Mix(2X), High Rox

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amfiSure qGreen Q-PCR Master Mix(2X), High Rox

Q5602-010 10x1ml
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amfiSure qGreen Q-PCR Master Mix(2X), High Rox

Q5602-050 50x1ml
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amfiSure qGreen Q-PCR Master Mix(2X), Low Rox

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amfiSure qGreen Q-PCR Master Mix(2X), Low Rox

Q5603-002 2x1ml
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amfiSure qGreen Q-PCR Master Mix(2X), Low Rox

Q5603-005 5x1ml
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amfiSure qGreen Q-PCR Master Mix(2X), Low Rox

Q5603-010 10x1ml
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amfiSure qGreen Q-PCR Master Mix(2X), Low Rox

Q5603-050 50x1ml
EUR 2004

amfiSure qProbe Q-PCR Master Mix(2X), No Rox

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Q5700-002 2x1ml
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amfiSure qProbe Q-PCR Master Mix(2X), No Rox

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amfiSure qProbe Q-PCR Master Mix(2X), High Rox

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Q5702-002 2x1ml
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Q5702-005 5x1ml
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amfiSure qProbe Q-PCR Master Mix(2X), High Rox

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amfiSure qProbe Q-PCR Master Mix(2X), High Rox

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Q5703-002 2x1ml
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Q5703-003 5x1ml
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Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL)

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Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn)

31015 2x1mL
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Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL)

31015-T 1mL
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31016-1 5x1mL
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31016-2 50x1mL
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Description: Minimum order quantity: 1 unit of 50x1mL

KiloGreen 2X qPCR MasterMix-ROX

MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 168

TaqProbe 2X qPCR MasterMix-ROX

MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R-XL 16 x 1.25 ml for 2000 reactions (20 ul)
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M-PCR-173L 960 reactions x 20 µl
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

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M-PCR-173S 192 reactions x 20 µl
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Evaluatie van de diagnostische prestaties van negen commerciële RT-PCR-kits voor de detectie van SARS-CoV-2 in Colombia 

The continuing SARS-CoV-2 pandemic has led to the design and improvement of a number of RT-PCR kits aimed to facilitate the fast scale-up of molecular testing for enormous screening. We evaluated the diagnostic efficiency of 9 business kits, which confirmed optimum efficiency and excessive discriminatory energy. Nonetheless, we noticed variations when it comes to sensitivity, specificity, and E gene Ct Values and talk about these ends in mild of the affect of SARS-CoV-2 genetic variability and its potential impression in present molecular diagnostic assays. This text is protected by copyright. All rights reserved.

Een zeer gevoelige en specifieke sonde-gebaseerde real-time PCR voor de detectie van Avibacterium paragallinarum in klinische monsters van pluimvee 

Avibacterium paragallinarum (traditionally known as Hemophilus paragallinarum) causes infectious coryza (IC), which is an acute respiratory illness of chickens. Lately, outbreaks of IC have been reported in Pennsylvania (PA) in broilers, layer pullets, and laying hens, inflicting important respiratory illness and manufacturing losses. A tentative prognosis of IC may be made based mostly on historical past, scientific indicators, and attribute gross lesions. Nonetheless, isolation and identification of the organism are required for a definitive prognosis. Main challenges with the bacteriological prognosis of A. paragallinarum embody that the organism is tough to isolate, slow-growing, and may solely be efficiently remoted through the acute stage of an infection and secondary bacterial infections are additionally frequent.

As there have been very restricted entire genomes of A. paragallinarum within the public databases, we carried out whole-genome sequencing (WGS) of PA isolates and based mostly on the WGS information evaluation; we designed a novel probe-based PCR assay concentrating on a extremely conserved sequence within the recN, the DNA restore protein gene of A. paragallinarum. The assay consists of an inner management, with a restrict of detection (LOD) of three.93 genomic copies. The PCR effectivity ranged between 90 and 97%, and diagnostic sensitivity of 98.5% in contrast with standard gel-based PCR.

The take a look at was extremely particular, and no cross-reactivity was noticed with different species of Avibacterium and a spread of different frequent poultry respiratory viral and bacterial pathogens. Actual-time PCR testing on 419 scientific samples from suspected flocks yielded 94 positives and 365 negatives in settlement with diagnostic bacterial culture-based detection. We additionally in contrast the recN PCR assay with a earlier HPG-2 based mostly real-time PCR assay which confirmed a PCR effectivity of 79%.

Gelijktijdige detectie en differentiatie van klinisch relevante recidiverende koorts Borrelia met semi-multiplex real-time PCR 

Bacterial vector-borne illnesses, together with Borrelia species, current a major diagnostic, scientific, and public well being problem attributable to their overlapping signs and the breadth of causative brokers and arthropod vectors. The relapsing fever (RF) borreliae embody each established and rising pathogens and are transmitted to people by gentle ticks, onerous ticks, or lice. We developed a real-time semi-multiplex PCR assay that detects a number of RF borreliae inflicting human sickness and classifies them into one in every of three teams. The teams are based mostly on genetic similarity and embody brokers of soft-tick relapsing fever (B. hermsii and others), the rising onerous tick transmitted pathogen B. miyamotoi, and the agent of louse-borne relapsing fever (B. recurrentis) The true-time PCR assay makes use of a single primer pair designed to amplify all identified pathogenic RF borreliae, and a number of TaqMan probes to permit for detection of and differentiation among the many three teams.

The assay detects all RF borreliae examined with an analytical restrict of detection beneath 15 genome equivalents per response. Thirty isolates of RF borreliae encompassing six species had been precisely recognized. Thirty-nine of 41 residual specimens (EDTA entire blood, serum, or plasma) from sufferers with RF had been detected and appropriately categorised. None of 42 scientific samples from sufferers with different infections and 46 tradition specimens from non-RF micro organism had been detected. The event of a single assay real-time PCR method will assist to enhance prognosis of RF by simplifying the choice of exams to assist in scientific administration of acutely ailing RF sufferers.

Overlevingsanalyse van de tijd tot SARS-CoV-2 PCR-negativering om PCR-recept bij gezondheidswerkers te optimaliseren: de Henares COVID-19-cohortstudie voor gezondheidswerkers

Targets: Reverse transcriptase PCR (RT-PCR) is taken into account the gold customary in diagnosing COVID-19. Contaminated healthcare employees don’t return to work till RT-PCR has demonstrated that the virus is now not current within the higher respiratory tract. The purpose of this research is to find out probably the most environment friendly time to carry out RT-PCR previous to healthcare employees’ reincorporation.

Supplies and strategies: This can be a cohort research of healthcare employees with RT-PCR-confirmed COVID-19. Knowledge had been collected utilizing the medical charts of healthcare employees and accomplished with a phone interview. Kaplan-Meier curves had been used to find out the affect of a number of variables on the time to RT-PCR negativisation. The impression of the variables on survival was assessed utilizing the Breslow take a look at. A Cox regression mannequin was developed together with the related variables.

Outcomes: 159 topics with a optimistic RT-PCR out of 374 employees with suspected COVID-19 had been included. The median time to negativisation was 25 days from symptom onset (IQR 20-35 days). Presence of IgG, dyspnoea, cough and throat ache had been related to important longer time to negativisation. Cox logistic regression was used to regulate for confounding variables. Solely dyspnoea and cough remained within the mannequin as important determinants of extended negativisation time. Adjusted HRs had been 0.68 (0.48-096) for dyspnoea and 0.61 (0.42-0.88) for dry cough.

Conclusions: RT-PCR through the first three weeks results in a excessive share of optimistic outcomes. Within the presence of respiratory signs, negativisation took almost 1 week extra. Those that developed antibodies wanted longer time to negativisate.

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M-PCR-156S 192 reactions x 20 µl
EUR 225.3
Description: lyophilised real-time PCR Master Mix for dual labeled probes

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Description: Disposable Pipets; Disposable Pipets, Serological, Misc

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G065-1 200 reactions
EUR 200.4

dNTP Mix (1 ml)

9002-1 each
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BOT2043 PK104
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BOT7630 EACH
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Semivolatile ISTD Mix (4000ppm) - 5.5mL Bottle

ECS-N-001 5.5ML
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ECS-N-018 5.5ML
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PCR-EZ D-PCR MASTER MIX

BS294 100RXN, 100prep
EUR 112.2

Egg Drop Syndrome One-Step PCR kit

Oneq-V026-100D 100T
EUR 1039.2

Egg Drop Syndrome One-Step PCR kit

Oneq-V026-150D 150T
EUR 1177.2

Egg Drop Syndrome One-Step PCR kit

Oneq-V026-50D 50T
EUR 861.6

amfiSure PCR Master Mix

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P0311-025 5X50 rxns
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P0311-050 10X50 rxns
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P0311-100 10x1ml
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P0311-125 15X50 rxns
EUR 333.6

amfiSure PCR Master Mix

P0311-200 20x1ml
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EUR 902.4

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P0311-500 100x50 rxns
EUR 1509.6

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P0331-010 2X50 rxns
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P0331-025 5X50 rxns
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P0331-050 10X50 rxns
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P0331-250 50x50 rxns
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Ready? PCR Mix-Dye

M1128-1000 each
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Ready? PCR Mix-Dye

M1128-200 each
EUR 229.2

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M1129-200 each
EUR 352.8

Robust Ready? PCR Mix

M1130-1000 each
EUR 738

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M1130-200 each
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Rigor? PCR Mix-Dye

M1133-200 each
EUR 385.2

Breeze? PCR Mix-Dye

M1135-200 each
EUR 320.4

Distant? PCR Mix-Dye

M1137-200 each
EUR 352.8

Image Distant? PCR Mix

M1138-200 each
EUR 385.2

Advance? PCR Mix-Dye

M1140-200 each
EUR 451.2

Fire Start? PCR Mix

M1141-200 each
EUR 451.2

Whole Blood PCR Mix

M1143-200 each
EUR 385.2

HotTaq Probe qPCR Mix (no ROX)

BT11002 250rxn
EUR 130.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (no ROX)

BT11102 250rxn
EUR 147.6
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen HRM Mix (no ROX)

BT11202 250rxn
EUR 147.6
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Volatile Organics Mix (Low Level)

60-BIG-MIX-200 1ML
EUR 116.28

Volatile Organics Mix (High Level)

60-BIG-MIX-2000 1ML
EUR 165.3

7077B PIPET SHRTY 1ML

7077B-1 250/pk
EUR 277.2
Description: Disposable Pipets; Disposable Pipets, Serological, Misc

7078B PIPET SHRTY 1ML

7078B-1 250/pk
EUR 288
Description: Disposable Pipets; Disposable Pipets, Serological, Misc

NVHO-1, 4 oz. bottle

NVHO-1 4 oz
EUR 42
Description: NVHO-1, 4 oz. bottle

Base/Neutral Spiking Mix (1000ppm) - 5.5mL Bottle

ECS-N-005 5.5ML
EUR 66.12

Semivolatile ISTD Mix (6) (2000ppm) - 5.5mL Bottle

ECS-N-201 5.5ML
EUR 150.48

The egg drop syndrome (76) RT PCR kit

RTq-V044-100D 100T
EUR 860.4

The egg drop syndrome (76) RT PCR kit

RTq-V044-150D 150T
EUR 969.6

The egg drop syndrome (76) RT PCR kit

RTq-V044-50D 50T
EUR 717.6

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173L 960 reactions x 20 µl
EUR 789.36
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173S 192 reactions x 20 µl
EUR 197.34
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

SensiFAST Lyo-Ready No-ROX Mix, 2x

BIO-11060 10 x 10ml Ask for price

SensiFAST Lyo-Ready No-ROX Mix, 2x

BIO-11061 5ml Ask for price

SensiFAST Probe No-ROX Mix, 50 mL

BIO-86050 5000 rxn Ask for price

SensiFAST SYBR No-ROX Mix, 50 mL

BIO-98050 5000 rxn Ask for price

SYBR Green qPCR Master Mix (No ROX)

HY-K0523 5 mL (500 rxns )
EUR 308.4

Accuris qMax Probe No Rox qPCR Mix

PR2001-N-1000 1 PC
EUR 561.29

PRICE DROP

110-012 4 x 1000 µl
EUR 159.6

PRICE DROP

110-012L 5x 4 x 1000 µl
EUR 621.6

PRICE DROP

110-012XL 10x 4 x 1000 µl
EUR 1126.8

MyTaq Blood PCR Mix, 2x

BIO-25053/S Sample Ask for price

MyTaq Blood PCR Mix, 2x

BIO-25054 250 Reactions Ask for price

mRNAExpress Tail PCR mix (5uM)

MR-TAIL-PR 100 ul
EUR 196.8

amfiSure Prime PCR Master Mix

P1311-025 5X50 rxns
EUR 193.2

amfiSure Prime PCR Master Mix

P1311-050 10X50 rxns
EUR 262.8

amfiSure Prime PCR Master Mix

P1311-125 15X50 rxns
EUR 327.6

amfiSure Prime PCR Master Mix

P1311-500 100x50 rxns
EUR 1485.6

amfiSure Advanced PCR Master Mix

P2311-025 5X50 rxns
EUR 198

amfiSure Advanced PCR Master Mix

P2311-050 10X50 rxns
EUR 267.6

amfiSure Advanced PCR Master Mix

P2311-125 15X50 rxns
EUR 338.4

amfiSure Advanced PCR Master Mix

P2311-250 50x50 rxns
EUR 902.4

amfiSure Advanced PCR Master Mix

P2311-500 100x50 rxns
EUR 1534.8

Robust Ready? PCR Mix-Dye

M1131-1000 each
EUR 738

Robust Ready? PCR Mix-Dye

M1131-200 each
EUR 255.6

Fire Start? PCR Mix-Dye

M1142-200 each
EUR 451.2

TuMinute PCR Clean-Up Kit 

P-1005
  • EUR 303.60
  • EUR 90.20
  • EUR 171.60
  • 100 Samples
  • 50 Samples
  • 100 Samples

AXYPREP MAG PCR NORMALIZER KIT- 1 ML - (SAMPLE SIZE)

MAG-PCR-NM-1 1/pk
EUR 87.6
Description: Bioscience Mag Beads; Magnetic PCR Normaliser

EPA Method 625 Base/Neutral Mix 1 (2000ppm) - 5.5mL Bottle

ECS-N-030 5.5ML
EUR 409.26

The egg drop syndrome (76) One-Step PCR kit

Oneq-V044-100D 100T
EUR 1039.2

The egg drop syndrome (76) One-Step PCR kit

Oneq-V044-150D 150T
EUR 1177.2

The egg drop syndrome (76) One-Step PCR kit

Oneq-V044-50D 50T
EUR 861.6

Base/Neutral Surrogate Mix (3) (1000ppm) - 5.5mL Bottle

ECS-N-002 5.5ML
EUR 52.44

Acid Extractables Surrogate Mix (3) (2000ppm) - 5.5mL Bottle

ECS-N-003 5.5ML
EUR 52.44

Base/Neutral Surrogate Mix (4) (1000ppm) - 5.5mL Bottle

ECS-N-022 5.5ML
EUR 66.12

Acid Extractables Surrogate Mix (4) (2000ppm) - 5.5mL Bottle

ECS-N-023 5.5ML
EUR 98.04

Semivolatile Surrogate Mix (6) (1000/2000ppm) - 5.5mL Bottle

ECS-N-027 5.5ML
EUR 88.92

Base/Neutral Surrogate Mix (3) (1000ppm) - 11.5mL Bottle

ECS-Z-002 11.5ML
EUR 98.04

Acid Extractables Surrogate Mix (3) (2000ppm) - 11.5mL Bottle

ECS-Z-003 11.5ML
EUR 98.04

Base/Neutral Surrogate Mix 4 component-10.5mL Bottle

ECS-Z-022 10.5ML
EUR 118.56

Acid Extractables Surrogate Mix 4 component-10.5mL Bottle

ECS-Z-023 10.5ML
EUR 186.96

AXYPREP MAG PCR CLEAN UP KIT- 1 ML - (SAMPLE SIZE)

MAG-PCR-CL-1 1/pk
EUR 69.6
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

PCR Tubes (50)

PCR-50 50
EUR 15
Description: To be used with the PractiChrom kits in conjuction with the PiCO Explorer. Quantity: 50 PCR tubes.

Bluetongue virus 1 PCR kit

PCR-V226-48R 50T
EUR 987.6

Bluetongue virus 1 PCR kit

PCR-V226-96R 100T
EUR 1335.6

Poliovirus 1 (PV1) PCR kit

PCR-H659-48R 50T
EUR 987.6

Poliovirus 1 (PV1) PCR kit

PCR-H659-96R 100T
EUR 1335.6

Psittacid herpesvirus 1 PCR kit

PCR-V014-48D 50T
EUR 543.6

Psittacid herpesvirus 1 PCR kit

PCR-V014-96D 100T
EUR 686.4

Equine herpesvirus 1 PCR kit

PCR-V144-48D 50T
EUR 543.6

Equine herpesvirus 1 PCR kit

PCR-V144-96D 100T
EUR 686.4

Bovine herpesvirus 1 PCR kit

PCR-V182-48D 50T
EUR 543.6

Bovine herpesvirus 1 PCR kit

PCR-V182-96D 100T
EUR 686.4

PCR Tube Strips no caps 10x12pcs.

E0030132882 EACH
EUR 165.3

Taq 2x PCR Mix 100 rxn

BA01501 100rxn
EUR 112.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq 2x PCR Mix 100 rxn

BA01503 100rxn
EUR 144
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq 2x PCR Mix 100 rxn

BA01507 100rxn
EUR 175.2
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

Taq 5x PCR Mix 12.5 mM

BT10402 250rxn
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

amfiSure ONE PCR Master Mix(2X)

P7000-005 5x1 ml
EUR 262.8

amfiSure ONE PCR Master Mix(2X)

P7000-010 10x1 ml
EUR 380.4

amfiSure ONE PCR Master Mix(2X)

P7000-050 50x1 ml
EUR 1453.2

amfiSure ONE PCR Master Mix(2X)

P7000-100 100x1 ml
EUR 2522.4

qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-360L 10 x 1,25 ml
EUR 720.29
Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-360S 2 x 1,25 ml
EUR 180.14
Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-362L 10 x 1,25 ml
EUR 720.29
Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-362S 2 x 1,25 ml
EUR 180.14
Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

Pesticide Mix 1 Containing 16 Compounds in Acetonitrile

SPXPR-1 1ML
EUR 282.72

qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-361L 10 x 1,25 ml
EUR 720.29
Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

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