Ontwikkeling en toepassing van een nieuwe
Ontwikkeling en toepassing van een nieuwe multiplex PCR-test voor de differentiatie van vier hemosporidische parasieten bij de kip Gallus gallus domesticus
Haemosporidian infections in home chickens (Gallus gallus domesticus) usually are not solely extensively prevalent but additionally trigger financial loss. Prognosis is often made by microscopic examination; nevertheless, the tactic has a number of drawbacks corresponding to requiring an skilled microscopist, being unreliable when parasitemia is low and being unable to precisely differentiate between co-infections from a number of parasite species. Subsequently, the present extent of haemosporidian infections could be underestimated and uncared for. We’ve got developed a novel multiplex PCR assay to concurrently detect and differentiate between 4 haemosporidian parasites: Leucocytozoon caulleryi, Leucocytozoon sabrazesi, Plasmodium juxtanucleare and Plasmodium gallinaceum. Primers within the current research particularly amplified the corresponding targets with no cross-species amplification detected.
The multiplex PCR exhibited a considerably higher detection price when put next with microscopic examination (p = 0.0001). The outcomes reveal that the detection price of multiplex PCR for L. sabrazesi, P. juxtanucleare, and P. gallinaceum are all higher than that of microscopic examination with p = 0.002, 0.0001 and 0.004, respectively. Co-infections had been additionally detected extra successfully by multiplex PCR. We utilized the present methodology to area samples originating from Nan, Prachinburi, and Chachoengsao Provinces. The present research revealed that constructive charges of haemosporidian parasites in chickens within the three research websites starting from 39.5%-93.8%. The current assay presents a timesaving choice for molecular analysis as an alternative of utilizing singleplex PCRs for detecting the parasites individually. Inside a single response, this assay could be a useful gizmo for the detection of avian haemosporidian parasites both single or below co-infection situations and for large-scale epidemiology research.
Recombinant Human Relaxin-3 |
|||
| MBS140915-1mg | MyBiosource | 1mg | EUR 2880 |
Recombinant Human Relaxin-3 |
|||
| MBS140915-5x1mg | MyBiosource | 5x1mg | EUR 12630 |
Relaxin-3, human recombinant |
|||
| 7195-10 | Biovision | each | EUR 248.4 |
Relaxin-3, human recombinant |
|||
| 7195-50 | Biovision | each | EUR 810 |
Relaxin-3 Human Recombinant |
|||
| rAP-4650 | Angio Proteomie | Inquiry | Ask for price |
INSL7 / Relaxin 3 (Human) |
|||
| 035-36 | PHOENIX PEPTIDE | 20 μg | EUR 160.92 |
INSL7 / Relaxin 3 (Human) |
|||
| 035-36A | PHOENIX PEPTIDE | 100 μg | EUR 604.8 |
Human Relaxin-3 ELISA Kit |
|||
| MBS2887639-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 4080 |
Human Relaxin-3 ELISA Kit |
|||
| MBS2887639-48StripWells | MyBiosource | 48-Strip-Wells | EUR 390 |
Human Relaxin-3 ELISA Kit |
|||
| MBS2887639-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2220 |
Human Relaxin-3 ELISA Kit |
|||
| MBS2887639-96StripWells | MyBiosource | 96-Strip-Wells | EUR 520 |
Human Relaxin-3 ELISA Kit |
|||
| MBS765502-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3900 |
Human Relaxin-3 ELISA Kit |
|||
| MBS765502-48StripWells | MyBiosource | 48-Strip-Wells | EUR 340 |
Human Relaxin-3 ELISA Kit |
|||
| MBS765502-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2045 |
Human Relaxin-3 ELISA Kit |
|||
| MBS765502-96StripWells | MyBiosource | 96-Strip-Wells | EUR 455 |
Human Relaxin-3,RLN-3 ELISA |
|||
| YLA4309HU-48T | Shanghai YL Biotech | 48T | Ask for price |
Human Relaxin-3,RLN-3 ELISA |
|||
| YLA4309HU-96T | Shanghai YL Biotech | 96T | Ask for price |
INSL7 / Relaxin 3 (Human) - Antibody |
|||
| H-035-36 | PHOENIX PEPTIDE | 50 μl | EUR 604.8 |
Human Relaxin 3 ELISA Kit |
|||
| MBS054925-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Human Relaxin 3 ELISA Kit |
|||
| MBS054925-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Human Relaxin 3 ELISA Kit |
|||
| MBS054925-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Human Relaxin 3 ELISA Kit |
|||
| MBS054925-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Human Relaxin 3 ELISA Kit |
|||
| EK5653 | SAB | 96 tests | EUR 599 |
Human Relaxin 3 ELISA Kit |
|||
| MBS8248392-5x96Tests | MyBiosource | 5x96Tests | EUR 2880 |
Human Relaxin 3 ELISA Kit |
|||
| MBS8248392-96Tests | MyBiosource | 96Tests | EUR 645 |
Human relaxin 3 ELISA Kit |
|||
| SL2396Hu | Sunlong | 96 Tests | EUR 468 |
Mouse Monoclonal anti-human Relaxin-3 |
|||
| hAP-0526 | Angio Proteomie | 100ug | EUR 250 |
Human Relaxin-3 Recombinant Protein |
|||
| 100-356 | ReliaTech | 25 µg | EUR 196.35 |
|
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain. |
|||
Human Relaxin-3 Recombinant Protein |
|||
| 100-356S | ReliaTech | 5 µg | EUR 92.4 |
|
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain. |
|||
Recombinant Human Relaxin-3 Protein |
|||
| PROTQ8WXF3-1 | BosterBio | 25ug | EUR 380.4 |
|
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain. |
|||
Human Relaxin-1 |
|||
| hAP-0525 | Angio Proteomie | 50ug | EUR 400 |
Human Relaxin-2 |
|||
| MBS691734-0005mg | MyBiosource | 0.005mg | EUR 300 |
Human Relaxin-2 |
|||
| MBS691734-0025mg | MyBiosource | 0.025mg | EUR 450 |
Human Relaxin-2 |
|||
| MBS691734-5x0025mg | MyBiosource | 5x0.025mg | EUR 1725 |
Human Relaxin 3 (RLN3) Protein |
|||
| abx680520-100g | Abbexa | 100 µg | EUR 225 |
ELISA Kit for Human Relaxin-3 |
|||
| E0189h | EIAab | 96T | EUR 387.4 |
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| AE22052HU-48T | Abebio | 48T | EUR 325 |
|
Description: Human (Homo sapiens) |
|||
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| AE22052HU-48Tests | Abebio | 48 Tests | EUR 325 |
|
Description: Human (Homo sapiens) |
|||
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| AE22052HU-96T | Abebio | 96T | EUR 610 |
|
Description: Human (Homo sapiens) |
|||
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| AE22052HU-96Tests | Abebio | 96 Tests | EUR 610 |
|
Description: Human (Homo sapiens) |
|||
Human Relaxin-3(RLN3) Elisa Kit |
|||
| EK713553 | AFG Bioscience LLC | 96 Wells | EUR 0.37 |
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| EK7514 | SAB | 96Т | EUR 799 |
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| KTE60790-48T | Abbkine | 48T | EUR 339 |
|
Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3. |
|||
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| KTE60790-96T | Abbkine | 96T | EUR 589 |
|
Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3. |
|||
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| KTE60790-96T5 | Abbkine | 96 T×5 | EUR 2629 |
|
Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3. |
|||
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| KTE60790-96T50 | Abbkine | 96 T×50 | EUR 24569 |
|
Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3. |
|||
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| MBS9714455-48Tests | MyBiosource | 48Tests | EUR 340 |
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| MBS9714455-5x96Tests | MyBiosource | 5x96Tests | EUR 1820 |
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| MBS9714455-96Tests | MyBiosource | 96Tests | EUR 500 |
Human Relaxin-3 (RLN3) ELISA Kit |
|||
| RK06347 | Abclonal | 96T | EUR 322.37 |
Relaxin-2 (Human) |
|||
| MBS408061-005mg | MyBiosource | 0.05mg | EUR 345 |
Relaxin-2 (Human) |
|||
| MBS408061-5x005mg | MyBiosource | 5x0.05mg | EUR 1500 |
Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7) |
|||
| MBS650130-0005mg | MyBiosource | 0.005mg | EUR 340 |
Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7) |
|||
| MBS650130-0025mg | MyBiosource | 0.025mg | EUR 490 |
Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7) |
|||
| MBS650130-5x0025mg | MyBiosource | 5x0.025mg | EUR 2055 |
Human RLN3/ Relaxin-3 ELISA Kit |
|||
| E2158Hu | Sunlong | 1 Kit | EUR 515 |
|
Description: UNQ6188/PRO20213,INSL7,RXN3,ZINS4,Insulin-like peptide INSL7,Prorelaxin H3,Insulin-like peptide 7 |
|||
Human RLN3(Relaxin-3) ELISA Kit |
|||
| EH0755 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
|||
Human RLN3 (Relaxin-3) ELISA Kit |
|||
| MBS2516020-10x96Tests | MyBiosource | 10x96Tests | EUR 4060 |
Human RLN3 (Relaxin-3) ELISA Kit |
|||
| MBS2516020-24Tests | MyBiosource | 24Tests | EUR 265 |
Human RLN3 (Relaxin-3) ELISA Kit |
|||
| MBS2516020-48Test | MyBiosource | 48Test | EUR 450 |
Human RLN3 (Relaxin-3) ELISA Kit |
|||
| MBS2516020-5x96Test | MyBiosource | 5x96Test | EUR 2265 |
Human RLN3 (Relaxin-3) ELISA Kit |
|||
| MBS2516020-96Tests | MyBiosource | 96Tests | EUR 535 |
Human RLN3(Relaxin-3) ELISA Kit |
|||
| RE1961H-48wells | Reed Biotech | 48 wells | EUR 125.55 |
Human RLN3(Relaxin-3) ELISA Kit |
|||
| RE1961H-48wellsplate | Reed Biotech | 48 wells plate | EUR 126 |
Human RLN3(Relaxin-3) ELISA Kit |
|||
| RE1961H-96wells | Reed Biotech | 96 wells | EUR 179.55 |
Human RLN3(Relaxin-3) ELISA Kit |
|||
| RE1961H-96wellsplate | Reed Biotech | 96 wells plate | EUR 180 |
Human Relaxin 3 (RLN3) ELISA Kit |
|||
| abx512123-96tests | Abbexa | 96 tests | EUR 904.8 |
Human Relaxin 3 (RLN3) ELISA Kit |
|||
| abx253713-96tests | Abbexa | 96 tests | EUR 562.5 |
Human Relaxin 3,RLN3 ELISA KIT |
|||
| JOT-EK7543Hu | Jotbody | 96 wells | Ask for price |
|
Description: Human |
|||
Human Relaxin 3,RLN3 ELISA KIT |
|||
| E7543Hu-1096T | Jiaxing Korain Biotech Ltd (BT Labs) | 10*96T | EUR 4122 |
Human Relaxin 3,RLN3 ELISA KIT |
|||
| E7543Hu-48wells | Jiaxing Korain Biotech Ltd (BT Labs) | 48 wells | EUR 300 |
Human Relaxin 3,RLN3 ELISA KIT |
|||
| E7543Hu-596T | Jiaxing Korain Biotech Ltd (BT Labs) | 5*96T | EUR 2061 |
Human Relaxin 3,RLN3 ELISA KIT |
|||
| E7543Hu-96wells | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | EUR 458 |
Human Relaxin 3, RLN3 ELISA Kit |
|||
| MBS1609027-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3460 |
Human Relaxin 3, RLN3 ELISA Kit |
|||
| MBS1609027-48StripWells | MyBiosource | 48-Strip-Wells | EUR 285 |
Human Relaxin 3, RLN3 ELISA Kit |
|||
| MBS1609027-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1750 |
Human Relaxin 3, RLN3 ELISA Kit |
|||
| MBS1609027-96StripWells | MyBiosource | 96-Strip-Wells | EUR 425 |
Nori® Human Relaxin-3 ELISA Kit |
|||
| GR111288 | Genorise Scientific | 96-well | EUR 461 |
Relaxin 2 (Human) |
|||
| 035-62 | PHOENIX PEPTIDE | 20 μg | EUR 261.36 |
Relaxin 2 (Human) |
|||
| 035-62A | PHOENIX PEPTIDE | 100 μg | EUR 469.8 |
Relaxin H2 (human) |
|||
| 4039910.02 | Bachem | 0.2 mg | EUR 455.07 |
Relaxin H2 (human) |
|||
| 4039910.1 | Bachem | 1 mg | EUR 1818.92 |
Relaxin H3 (human) |
|||
| 4039569.02 | Bachem | 0.2 mg | EUR 455.07 |
Relaxin H3 (human) |
|||
| 4039569.1 | Bachem | 1 mg | EUR 1818.92 |
Relaxin H2 (human) |
|||
| H-6784.0200 | Bachem | 0.2mg | EUR 741.6 |
|
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net |
|||
Relaxin H2 (human) |
|||
| H-6784.1000 | Bachem | 1.0mg | EUR 2853.6 |
|
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net |
|||
Relaxin H3 (human) |
|||
| H-7322.0200 | Bachem | 0.2mg | EUR 741.6 |
|
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net |
|||
Relaxin H3 (human) |
|||
| H-7322.1000 | Bachem | 1.0mg | EUR 2853.6 |
|
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net |
|||
Recombinant Human Relaxin-3 (RLN3), partial |
|||
| MBS7094488-005mgBaculovirus | MyBiosource | 0.05mg(Baculovirus) | EUR 885 |
Recombinant Human Relaxin-3 (RLN3), partial |
|||
| MBS7094488-005mgEColi | MyBiosource | 0.05mg(E-Coli) | EUR 510 |
Recombinant Human Relaxin-3 (RLN3), partial |
|||
| MBS7094488-02mgEColi | MyBiosource | 0.2mg(E-Coli) | EUR 665 |
Recombinant Human Relaxin-3 (RLN3), partial |
|||
| MBS7094488-02mgYeast | MyBiosource | 0.2mg(Yeast) | EUR 945 |
Recombinant Human Relaxin-3 (RLN3), partial |
|||
| MBS7094488-05mgEColi | MyBiosource | 0.5mg(E-Coli) | EUR 710 |
ELISA kit for Human Relaxin-3 (RLN3) |
|||
| KTE60790-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2538 |
|
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
|||
INSL7 / Relaxin 3 (Human) - RIA Kit |
|||
| RK-035-36 | PHOENIX PEPTIDE | 125 tubes | EUR 1061.64 |
Relaxin 2 (Human) - Antibody |
|||
| H-035-62 | PHOENIX PEPTIDE | 100 μl | EUR 604.8 |
Relaxin-3 |
|||
| AP87248 | SAB | 1mg | EUR 2640 |
Relaxin-3 |
|||
| AP87370 | SAB | 1mg | EUR 2640 |
Relaxin-3 |
|||
| AP87734 | SAB | 1mg | EUR 2640 |
Relaxin-3 |
|||
| AP88315 | SAB | 1mg | EUR 2640 |
Human Relaxin 3-RLN3-ELISA Kit |
|||
| QY-E01376 | Qayee Biotechnology | 96T | EUR 400 |
Human RLN3 (Relaxin 3) ELISA Kit |
|||
| MBS8819812-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3130 |
Human RLN3 (Relaxin 3) ELISA Kit |
|||
| MBS8819812-48StripWells | MyBiosource | 48-Strip-Wells | EUR 350 |
Human RLN3 (Relaxin 3) ELISA Kit |
|||
| MBS8819812-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1710 |
Human RLN3 (Relaxin 3) ELISA Kit |
|||
| MBS8819812-96StripWells | MyBiosource | 96-Strip-Wells | EUR 445 |
Human Relaxin 3 PicoKine ELISA Kit |
|||
| MBS177627-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3935 |
Human Relaxin 3 PicoKine ELISA Kit |
|||
| MBS177627-96StripWells | MyBiosource | 96-Strip-Wells | EUR 855 |
RLN3 (untagged)-Human relaxin 3 (RLN3) |
|||
| SC305868 | Origene Technologies GmbH | 10 µg | Ask for price |
Anti- Relaxin-3 receptor 2 Antibody |
|||
| GWB-EDDDF9 | GenWay Biotech | 0.05 ml | Ask for price |
Anti- Relaxin-3 receptor 2 Antibody |
|||
| GWB-214500 | GenWay Biotech | 0.05 ml | Ask for price |
ELISA kit for Human RLN3 (Relaxin-3) |
|||
| E-EL-H1652 | Elabscience Biotech | 1 plate of 96 wells | EUR 640.8 |
|
Description: A sandwich ELISA kit for quantitative measurement of Human RLN3 (Relaxin-3) in samples from Serum, Plasma, Cell supernatant |
|||
Relaxin 3 (BDelta23-27) R (Human) |
|||
| 036-20 | PHOENIX PEPTIDE | 100 μg | EUR 461.16 |
EasyPro Human Relaxin-3(RLN3)ELISA Kit |
|||
| FY-EH1961S | Feiyuebio | each | EUR 520 |
|
Description: 18.75 pg/mL |
|||
RLN3 Relaxin-3 Human Recombinant Protein |
|||
| PROTQ8WXF3 | BosterBio | Regular: 25ug | EUR 250 |
|
Description: Relaxin-3 Human Recombinant produced in E. coli is a disulfide-linked heterodimeric, non-glycosylated, polypeptide chain containing 24 amino acids for A chain and 27 amino acids for B chain and having a molecular mass of 2.5kDa for A chain and 3kDa for B chain. The Relaxin-3 is purified by proprietary chromatographic techniques. |
|||
INSL7 / Relaxin 3 (Human) - Cy5 Labeled |
|||
| FC5-035-36 | PHOENIX PEPTIDE | 1 nmol | EUR 756 |
Human Relaxin-3 Recombinant Protein Lyophilized |
|||
| IHURLXN3RLY5UG | Innovative research | each | EUR 341 |
|
Description: Human Relaxin-3 Recombinant Protein Lyophilized |
|||
Human Relaxin-3 Recombinant Protein Lyophilized |
|||
| MBS8433281-INQUIRE | MyBiosource | INQUIRE | Ask for price |
INSL7 / Relaxin 3 (Human) - Purified IgG Antibody |
|||
| G-035-36 | PHOENIX PEPTIDE | 200 μg | EUR 604.8 |
Recombinant Human Relaxin-2 |
|||
| MBS146516-0005mg | MyBiosource | 0.005mg | EUR 240 |
Recombinant Human Relaxin-2 |
|||
| MBS146516-0025mg | MyBiosource | 0.025mg | EUR 310 |
De associatie van leeftijd, geslacht en RT-PCR-resultaten met het aantal lymfocyten en neutrofielen in SARS-CoV-2-infectie: een dwarsdoorsnede-analyse van 1450 Iraanse patiënten met COVID-19
Containment of pandemic infections primarily relies on immediate identification of carriers, achievable via strict surveillance and truthful diagnostic testing. Though molecular identification of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the gold customary methodology, its low sensitivity and lengthy turnaround time are amongst main issues. On this retrospective single-center research, we reviewed the outcomes of the lymphocyte and neutrophil counts of 1450 Iranian sufferers with coronavirus illness 2019 (COVID-19) recruited at Baqiyatallah Hospital, Tehran, Iran. Of 1450 sufferers, 439 instances (30.3%) had been polymerase chain response (PCR) unfavourable; additional emphasizing that getting unfavourable molecular testing shouldn’t be as dependable as a constructive consequence. Whereas the lymphocyte depend in instances with lower than 50 years previous was 1.8×103/µL (1.2-2.5), it was 1.47×103/µL (0.84-2.16) within the older group (p<0.001). Additionally, males skilled decrease lymphocytes as in comparison with girls (1.53×103/µL vs 1.76×103/µL; p=0.002). Of specific curiosity, the lymphocyte depend within the PCR-negative instances was 1.77×103/µL (0.98-2.45) which was considerably larger than its depend of their constructive counterparts (1.53×103/µL; p=0.004).
Not like lymphocytes, intercourse and PCR didn’t considerably have an effect on the variety of neutrophils. The percentages ratio for neutrophilia in sufferers aged older than 50, both with a unfavourable or a constructive PCR, was 2.46 and a pair of.23, suggesting previous age as probably the most important related issue. The variety of lymphocytes together with elevated neutrophil depend could in all probability function easy, speedy, and economical biomarkers, and are seemingly acceptable objects that needs to be taken into consideration within the identification of sufferers with COVID-19, particularly these aged greater than 50.
Analyse en voorspelling van wereldwijde real-time RT-PCR-primers en probes voor SARS-CoV-2
Speedy exams for lively SARS-CoV-2 infections depend on reverse transcription polymerase chain response (RT-PCR). RT-PCR makes use of reverse transcription of RNA into complementary DNA (cDNA) and amplification of particular DNA (primer and probe) targets utilizing polymerase chain response (PCR). The expertise makes speedy and particular identification of the virus doable primarily based on sequence homology of nucleic acid sequence and is way sooner than tissue tradition or animal cell fashions. Nonetheless the method can lose sensitivity over time because the virus evolves and the goal sequences diverge from the selective primer sequences. Totally different primer sequences have been adopted in numerous geographic areas.
As we depend on these current RT-PCR primers to trace and handle the unfold of the Coronavirus, it’s crucial to know how SARS-CoV-2 mutations, over time and geographically, diverge from current primers used right this moment. On this research, we analyze the efficiency of the SARS-CoV-2 primers in use right this moment by measuring the variety of mismatches between primer sequence and genome targets over time and spatially. We discover that there’s a rising variety of mismatches, a rise by 2% monthly, in addition to a excessive specificity of virus primarily based on geographic location
SARS-CoV-2 E-genvariant verandert analytische gevoeligheidskenmerken van virale detectie met behulp van een commerciële RT-PCR-test
Diagnostic assays for detecting SARS-CoV-2 are important for affected person administration, an infection prevention, and the general public well being response for COVID-19. The efficacy and reliability of those assays are of paramount significance in each monitoring and controlling unfold of the virus. Actual-time RT-PCR assays depend on a hard and fast genetic sequence for primers and probe binding. Mutations can probably alter the accuracy of those assays and result in unpredictable analytical efficiency traits and false-negative outcomes. Herein, we determine a G-to-U transversion (nucleotide 26372) within the SARS-CoV-2 E gene in three specimens with diminished viral detection effectivity utilizing a extensively out there industrial assay. Additional evaluation of the general public GISAID repository led to the identification of 18 further genomes with this mutation, which replicate 5 impartial mutational occasions. This work helps using dual-target assays to cut back the variety of false-negative PCR outcomes.
Argonaute geïntegreerd PCR-systeem met één buis maakt overgevoelige detectie van zeldzame mutaties mogelijk
Technological advances in uncommon DNA mutations detection have revolutionized the analysis and monitoring of tumors, however they’re nonetheless restricted by the dearth of supersensitive and high-coverage procedures for figuring out low-abundance mutations. Right here, we describe a single-tube, multiplex PCR-based system, A-Star, that entails a hyperthermophilic Argonaute from Pyrococcus furiosus (PfAgo) for extremely environment friendly detection of uncommon mutations helpful from its compatibility with DNA polymerase. This novel method makes use of a particular information design technique to permit PfAgo selective cleavage with single-nucleotide decision at 94°C, thus largely eliminating wild-type DNA within the denaturation step and effectively amplifying uncommon mutant DNA through the PCR course of.
The built-in single-tube system achieved nice effectivity for enriching uncommon mutations in contrast with a divided system separating the cleavage and amplification. Thus, A-Star allows simple detection and quantification of 0.01% uncommon mutations with ≥5500-fold enhance in effectivity. The feasibility of A-Star was additionally demonstrated for detecting oncogenic mutations in strong tumor tissues and blood samples. Remarkably, A-Star achieved simultaneous detection of a number of oncogenes via a easy single-tube response by orthogonal guide-directed particular cleavage. This research demonstrates a supersensitive and speedy nucleic acid detection system with promising potential for each analysis and therapeutic functions.
SYBR Green qPCR Master Mix (No ROX) |
|||
| C0006-5mg | TargetMol Chemicals | 5mg | Ask for price |
|
Description: SYBR Green qPCR Master Mix (No ROX) |
|||
SYBR Green qPCR Master Mix (No ROX) |
|||
| HY-K0523 | MedChemExpress | 5 mL (500 rxns ) | EUR 308.4 |
SYBR Green qPCR Master Mix (Low ROX) |
|||
| C0008-10mg | TargetMol Chemicals | 10mg | Ask for price |
|
Description: SYBR Green qPCR Master Mix (Low ROX) |
|||
SYBR Green qPCR Master Mix (Low ROX) |
|||
| C0008-1g | TargetMol Chemicals | 1g | Ask for price |
|
Description: SYBR Green qPCR Master Mix (Low ROX) |
|||
SYBR Green qPCR Master Mix (Low ROX) |
|||
| C0008-1mg | TargetMol Chemicals | 1mg | Ask for price |
|
Description: SYBR Green qPCR Master Mix (Low ROX) |
|||
SYBR Green qPCR Master Mix (Low ROX) |
|||
| C0008-50mg | TargetMol Chemicals | 50mg | Ask for price |
|
Description: SYBR Green qPCR Master Mix (Low ROX) |
|||
SYBR Green qPCR Master Mix (Low ROX) |
|||
| C0008-5mg | TargetMol Chemicals | 5mg | Ask for price |
|
Description: SYBR Green qPCR Master Mix (Low ROX) |
|||
SYBR Green qPCR Master Mix (Low ROX) |
|||
| HY-K0522 | MedChemExpress | 5 mL (500 rxns ) | EUR 308.4 |
SYBR Green qPCR Master Mix (High ROX) |
|||
| C0007-10mg | TargetMol Chemicals | 10mg | Ask for price |
|
Description: SYBR Green qPCR Master Mix (High ROX) |
|||
SYBR Green qPCR Master Mix (High ROX) |
|||
| C0007-1g | TargetMol Chemicals | 1g | Ask for price |
|
Description: SYBR Green qPCR Master Mix (High ROX) |
|||
SYBR Green qPCR Master Mix (High ROX) |
|||
| C0007-1mg | TargetMol Chemicals | 1mg | Ask for price |
|
Description: SYBR Green qPCR Master Mix (High ROX) |
|||
SYBR Green qPCR Master Mix (High ROX) |
|||
| C0007-50mg | TargetMol Chemicals | 50mg | Ask for price |
|
Description: SYBR Green qPCR Master Mix (High ROX) |
|||
SYBR Green qPCR Master Mix (High ROX) |
|||
| C0007-5mg | TargetMol Chemicals | 5mg | Ask for price |
|
Description: SYBR Green qPCR Master Mix (High ROX) |
|||
SYBR Green qPCR Master Mix (High ROX) |
|||
| HY-K0521 | MedChemExpress | 1 mL (100 rxns) | EUR 135.6 |
2x SYBR Green qPCR Master Mix |
|||
| B21202 | Bimake | 5 mL | EUR 268.8 |
|
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
|||
2x SYBR Green qPCR Master Mix |
|||
| B21203 | Bimake | 25 mL | EUR 1027.2 |
|
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
|||
ig SYBR Green qPCR 2X Master Mix |
|||
| 3354 | Intact Genomics | 200 | EUR 92 |
|
Description: Intact Genomics SYBR Green qPCR 2X Master Mix master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification. |
|||
SYBR Green qPCR Master Mix |
|||
| HY-K0501 | MedChemExpress | 5 mL (500 rxns) | EUR 315.6 |
Maxima SYBR Green/ROX qPCR Master Mix - 200 x 25 µL rxns |
|||
| THK0221 | Westburg | each | Ask for price |
Maxima SYBR Green/ROX qPCR Master Mix - 1,000 x 25 µL rxns |
|||
| THK0222 | Westburg | each | Ask for price |
Maxima SYBR Green/ROX qPCR Master Mix - 4,000 x 25 µL rxns |
|||
| THK0223 | Westburg | each | EUR 3324.5 |
AceQ qPCR SYBR® Green Master Mix |
|||
| Q111-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
|||
| Q111-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 775.2 |
2X SYBR Green Fast qPCR Mix with High ROX |
|||
| RK21201 | Abclonal | 50mL | EUR 109 |
ViPrimePLUS Taq qPCR Green Master Mix I with ROX, 150rxns |
|||
| QLMM12-R | Vivantis | each | Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix I with ROX, 150rxns |
|||
| QLMM02-R | Vivantis | each | Ask for price |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|||
| Q321-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|||
| Q321-02-500rxns | Vazyme | 500 rxns | EUR 124.83 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|||
| Q321-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 543.5 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|||
| Q321-03-2500rxns | Vazyme | 2,500 rxns | EUR 562.79 |
Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 200 x 25 µL rxns |
|||
| THK0251 | Westburg | each | EUR 232.17 |
Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 1,000 x 25 µL rxns |
|||
| THK0252 | Westburg | each | EUR 1046.4 |
Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 4,000 x 25 µL rxns |
|||
| THK0253 | Westburg | each | Ask for price |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|||
| 17270-1mL | AAT Bioquest | 1 mL | EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|||
| 17271 | AAT Bioquest | 5 mL | EUR 508 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|||
| 17271-5mL | AAT Bioquest | 5 mL | EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|||
| 17272 | AAT Bioquest | 1 mL | EUR 203 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|||
| 17272-1mL | AAT Bioquest | 1 mL | EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|||
| 17273 | AAT Bioquest | 5 mL | EUR 508 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|||
| 17273-5mL | AAT Bioquest | 5 mL | EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|||
| 17274 | AAT Bioquest | 1 mL | EUR 203 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|||
| 17274-1mL | AAT Bioquest | 1 mL | EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|||
| 17275 | AAT Bioquest | 5 mL | EUR 508 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|||
| 17275-5mL | AAT Bioquest | 5 mL | EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
BioEasy Master Mix (SYBR Green, No ROX) |
|||
| TRI-B25L1D | TRI Biotech | 200T | EUR 403.2 |
ViPrimePLUS Taq qPCR Green Master Mix I with Low ROX, 150rxns |
|||
| QLMM12-LR | Vivantis | each | Ask for price |
BioEasy Master Mix (SYBR Green, low ROX) |
|||
| TRI-B25L1C | TRI Biotech | 200T | EUR 403.2 |
BioEasy Master Mix (SYBR Green, high ROX) |
|||
| TRI-B25L1B | TRI Biotech | 200T | EUR 403.2 |
ViPrimePLUS AtTaq qPCR Green Master Mix I with Low ROX, 150rxns |
|||
| QLMM02-LR | Vivantis | each | Ask for price |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|||
| Q421-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|||
| Q421-02-500rxns | Vazyme | 500 rxns | EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|||
| Q421-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 634 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|||
| Q421-03-2500rxns | Vazyme | 2,500 rxns | EUR 656.51 |
ViPrimePLUS Taq qPCR Green Master Mix II with ROX, 150rxns |
|||
| QLMM17-R | Vivantis | each | Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix II with ROX, 150rxns |
|||
| QLMM07-R | Vivantis | each | Ask for price |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|||
| 17276 | AAT Bioquest | 1 mL | EUR 203 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|||
| 17276-1mL | AAT Bioquest | 1 mL | EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|||
| 17277 | AAT Bioquest | 5 mL | EUR 508 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|||
| 17277-5mL | AAT Bioquest | 5 mL | EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|||
| 17278 | AAT Bioquest | 1 mL | EUR 203 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|||
| 17278-1mL | AAT Bioquest | 1 mL | EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|||
| 17279 | AAT Bioquest | 5 mL | EUR 508 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|||
| 17279-5mL | AAT Bioquest | 5 mL | EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|||
| 17280 | AAT Bioquest | 1 mL | EUR 203 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|||
| 17280-1mL | AAT Bioquest | 1 mL | EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|||
| 17281 | AAT Bioquest | 5 mL | EUR 508 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|||
| 17281-5mL | AAT Bioquest | 5 mL | EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
2X SYBR Green Fast qPCR Mix No Rox |
|||
| RK21200 | Abclonal | 50mL | EUR 109 |
ViPrimePLUS Taq qPCR Green Master Mix II with Low ROX, 150rxns |
|||
| QLMM17-LR | Vivantis | each | Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix II with Low ROX, 150rxns |
|||
| QLMM07-LR | Vivantis | each | Ask for price |
2X SYBR Green Fast qPCR Mix with Low ROXII |
|||
| RK21202 | Abclonal | 5mL | EUR 1000.08 |
Maxima SYBR Green/Fluorescein qPCR Master Mix - 200 x 25 µL rxns |
|||
| THK0241 | Westburg | each | Ask for price |
Maxima SYBR Green/Fluorescein qPCR Master Mix - 1,000 x 25 µL rxns |
|||
| THK0242 | Westburg | each | Ask for price |
Maxima SYBR Green/Fluorescein qPCR Master Mix - 4,000 x 25 µL rxns |
|||
| THK0243 | Westburg | each | Ask for price |
HotStart™ 2X Green qPCR Master Mix |
|||
| K1070-25 | ApexBio | 25ml | EUR 392 |
|
Description: Reverse Transcription & PCR|qPCR |
|||
HotStart™ 2X Green qPCR Master Mix |
|||
| K1070-5 | ApexBio | 5ml | EUR 124 |
|
Description: Reverse Transcription & PCR|qPCR |
|||
HotStart™ 2X Green qPCR Master Mix |
|||
| K1070-50 | ApexBio | 50ml | EUR 768 |
|
Description: Reverse Transcription & PCR|qPCR |
|||
Genious 2X SYBR Green Fast qPCR Mix (No ROX) |
|||
| RK21205 | Abclonal | 1 ml | EUR 324.28 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|||
| Q331-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|||
| Q331-02-500rxns | Vazyme | 500 rxns | EUR 124.83 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|||
| Q331-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 543.5 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|||
| Q331-03-2500rxns | Vazyme | 2,500 rxns | EUR 562.79 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|||
| Q341-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 120.55 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|||
| Q341-02-500rxns | Vazyme | 500 rxns | EUR 124.83 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|||
| Q341-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 543.5 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|||
| Q341-03-2500rxns | Vazyme | 2,500 rxns | EUR 562.79 |
rEVAlution 2X qPCR Master Mix with separate ROX |
|||
| MBS308019-1000Reactions | MyBiosource | 1000Reactions | EUR 650 |
rEVAlution 2X qPCR Master Mix with separate ROX |
|||
| MBS308019-100Reactions | MyBiosource | 100Reactions | EUR 175 |
rEVAlution 2X qPCR Master Mix with separate ROX |
|||
| MBS308019-500Reactions | MyBiosource | 500Reactions | EUR 385 |
2X qPCR Probe Master Mix with Separate ROX |
|||
| MBS308018-1000Reactions | MyBiosource | 1000Reactions | EUR 1150 |
2X qPCR Probe Master Mix with Separate ROX |
|||
| MBS308018-100Reactions | MyBiosource | 100Reactions | EUR 210 |
2X qPCR Probe Master Mix with Separate ROX |
|||
| MBS308018-2500Reactions | MyBiosource | 2500Reactions | EUR 5350 |
2X qPCR Probe Master Mix with Separate ROX |
|||
| MBS308018-500Reactions | MyBiosource | 500Reactions | EUR 405 |
2×SYBR Green qPCR Mix(including ROX) |
|||
| E37F022-10x5mL | EnoGene | 10×5mL | EUR 1360 |
|
Description: Fluorescent quantitative PCR kits |
|||
2×SYBR Green qPCR Mix(including ROX) |
|||
| E37F022-5mL | EnoGene | 5mL | EUR 227 |
|
Description: Fluorescent quantitative PCR kits |
|||
2×SYBR Green qPCR Mix (including ROX) |
|||
| MBS8579841-200Tests | MyBiosource | 200Tests | EUR 285 |
2×SYBR Green qPCR Mix (including ROX) |
|||
| MBS8579841-50Tests | MyBiosource | 50Tests | EUR 180 |
2×SYBR Green qPCR Mix (including ROX) |
|||
| MBS8579841-5x200Tests | MyBiosource | 5x200Tests | EUR 1230 |
2×SYBR Green qPCR Mix (including ROX) |
|||
| MBS8579841-5x50Tests | MyBiosource | 5x50Tests | EUR 770 |
BioEasy Master Mix (SYBR Green) |
|||
| TRI-B25L1 | TRI Biotech | 200T | EUR 403.2 |
HotStart™ 2X FAST Green qPCR Master Mix |
|||
| K1171-25 | ApexBio | 25ml | EUR 424 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
|||
HotStart™ 2X FAST Green qPCR Master Mix |
|||
| K1171-5 | ApexBio | 5ml | EUR 132 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
|||
HotStart™ 2X FAST Green qPCR Master Mix |
|||
| K1171-50 | ApexBio | 50ml | EUR 826.4 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
|||
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|||
| Q431-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|||
| Q431-02-500rxns | Vazyme | 500 rxns | EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|||
| Q431-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 634 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|||
| Q431-03-2500rxns | Vazyme | 2,500 rxns | EUR 656.51 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|||
| Q441-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|||
| Q441-02-500rxns | Vazyme | 500 rxns | EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|||
| Q441-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 634 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|||
| Q441-03-2500rxns | Vazyme | 2,500 rxns | EUR 656.51 |
HotStart™ Universal 2X Green qPCR Master Mix |
|||
| K1170-25 | ApexBio | 25ml | EUR 392 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
|||
HotStart™ Universal 2X Green qPCR Master Mix |
|||
| K1170-5 | ApexBio | 5ml | EUR 124 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
|||
HotStart™ Universal 2X Green qPCR Master Mix |
|||
| K1170-50 | ApexBio | 50ml | EUR 752 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
|||
Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed) |
|||
| RK21206 | Abclonal | 1 ml | EUR 76.3 |
ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with ROX, 150rxns |
|||
| QLMM14-R | Vivantis | each | Ask for price |
Genious 2X SYBR Green Fast qPCR Mix (High ROX Premixed) |
|||
| RK21207 | Abclonal | 1 ml | EUR 21.26 |
ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix I with ROX, 150rxns |
|||
| QLMM04-R | Vivantis | each | Ask for price |
ABsolute SYBR Green QPCR Mix + ROX 800 rxns |
|||
| AB-1162/B | Westburg | each | Ask for price |
ABsolute SYBR Green QPCR Mix + ROX 200 rxns |
|||
| AB-1163 | Westburg | each | EUR 543.37 |
SG/ROX qPCR Master Mix (2x) |
|||
| E0400-01 | EURx | 100reactions | EUR 55.59 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
|||
SG/ROX qPCR Master Mix (2x) |
|||
| E0400-02 | EURx | 200reactions | EUR 106.82 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
|||
SG/ROX qPCR Master Mix (2x) |
|||
| E0400-03 | EURx | 1000reactions | EUR 467.61 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
|||
SYBR Green cRNA PCR Master Mix |
|||
| SGM002 | Signosis | 2 ml | EUR 174 |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
|||
| 4302A | Unibiotest | 1 mL | EUR 80 |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
|||
| 4302B | Unibiotest | 5*1 mL | EUR 250 |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
|||
| 4302C | Unibiotest | 50*1 mL | EUR 1400 |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
|||
| 4302D | Unibiotest | 100*1 mL | EUR 1600 |
EnoGene Green qPCR Master Mix |
|||
| E31M001 | EnoGene | 500 reaction | EUR 75 |
ChamQ SYBR qPCR Master Mix |
|||
| Q311-03-2500rxns | Vazyme | 2,500 rxns | EUR 562.79 |
ChamQ SYBR qPCR Master Mix |
|||
| Q311-00-100rxns | Vazyme | 100 rxns | EUR 1.09 |
ChamQ SYBR qPCR Master Mix |
|||
| Q311-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 120.55 |
ChamQ SYBR qPCR Master Mix |
|||
| Q311-02-500rxns | Vazyme | 500 rxns | EUR 124.83 |
ChamQ SYBR qPCR Master Mix |
|||
| Q311-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 543.5 |
HotStart™ Universal 2X FAST Green qPCR Master Mix |
|||
| K1172-25 | ApexBio | 25ml | EUR 424 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
|||
HotStart™ Universal 2X FAST Green qPCR Master Mix |
|||
| K1172-5 | ApexBio | 5ml | EUR 132 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
|||
HotStart™ Universal 2X FAST Green qPCR Master Mix |
|||
| K1172-50 | ApexBio | 50ml | EUR 826.4 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
|||
1ml PrecisionPLUS qPCR Master Mix with ROX |
|||
| Z-PPLUS-R-1ML | Novacyt Group | 1ml | EUR 81 |
|
Description: Precision®PLUS |
|||
ABsolute QPCR SYBR Green Low Rox Mix 800x50µl |
|||
| AB-1322/B | Westburg | each | Ask for price |
ABsolute QPCR SYBR Green Low Rox Mix 200x50µl |
|||
| AB-1323/A | Westburg | each | EUR 659.45 |
ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with Low ROX, 150rxns |
|||
| QLMM14-LR | Vivantis | each | Ask for price |
ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix I with Low ROX, 150rxns |
|||
| QLMM04-LR | Vivantis | each | Ask for price |
Apex qPCR GREEN Master Mix |
|||
| 42-116PG | Genesee Scientific | 400 x 25µl Reactions/Unit | EUR 341.44 |
|
Description: 2X, Without ROX, 5ml |
|||
Apex qPCR GREEN Master Mix |
|||
| 42-117PG | Genesee Scientific | 4000 x 25µl Reactions/Unit | EUR 2926.74 |
|
Description: 2X, Without ROX, 50ml |
|||
Apex qPCR GREEN Master Mix |
|||
| 42-118PG | Genesee Scientific | 400 x 25µl Reactions/Unit | EUR 338.88 |
|
Description: 2X, Low ROX, 5ml |
|||
Apex qPCR GREEN Master Mix |
|||
| 42-119PG | Genesee Scientific | 4000 x 25µl Reactions/Unit | EUR 2926.74 |
|
Description: 2X, Low ROX, 50ml |
|||
Apex qPCR GREEN Master Mix |
|||
| 42-120PG | Genesee Scientific | 400 x 25µl Reactions/Unit | EUR 341.44 |
|
Description: 2X, High ROX, 5ml |
|||
Apex qPCR GREEN Master Mix |
|||
| 42-121PG | Genesee Scientific | 4000 x 25µl Reactions/Unit | EUR 3095.57 |
|
Description: 2X, High ROX, 50ml |
|||
Fast SG/ROX qPCR Master Mix (2x) |
|||
| E0413-01 | EURx | 100reactions | EUR 61.04 |
|
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
|||
Fast SG/ROX qPCR Master Mix (2x) |
|||
| E0413-02 | EURx | 200reactions | EUR 116.63 |
|
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
|||
Fast SG/ROX qPCR Master Mix (2x) |
|||
| E0413-03 | EURx | 1000reactions | EUR 534.1 |
|
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
|||
SG/ROX onTaq qPCR Master Mix (2x) |
|||
| E0407-01 | EURx | 100reactions | EUR 54.5 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains onTaq polymerase and ROX dye |
|||
SG/ROX onTaq qPCR Master Mix (2x) |
|||
| E0407-02 | EURx | 200reactions | EUR 104.64 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains onTaq polymerase and ROX dye |
|||
SG/ROX onTaq qPCR Master Mix (2x) |
|||
| E0407-03 | EURx | 1000reactions | EUR 455.62 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains onTaq polymerase and ROX dye |
|||
ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with ROX, 150rxns |
|||
| QLMM18-R | Vivantis | each | Ask for price |
ViPrimePLUS Taq qPCR Master Mix with ROX, 150rxns |
|||
| QLMM11-R | Vivantis | each | Ask for price |
ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix II with ROX, 150rxns |
|||
| QLMM08-R | Vivantis | each | Ask for price |
ViPrimePLUS AtTaq qPCR Master Mix with ROX, 150rxns |
|||
| QLMM01-R | Vivantis | each | Ask for price |
ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns |
|||
| QLMM10 | Vivantis | each | Ask for price |
1ml Precision FAST qPCR Master Mix with ROX |
|||
| Z-PFAST-R-1ML | Novacyt Group | 1ml | EUR 90 |
|
Description: Precision®FAST |
|||
2ml Precision FAST qPCR Master Mix with ROX |
|||
| Z-PFAST-R-2ML | Novacyt Group | 2ml | EUR 161 |
|
Description: Precision®FAST |
|||
Genious 2X SYBR Green Fast qPCR Mix |
|||
| RK21204 | Abclonal | 1 mL | EUR 76.3 |
ChamQ SYBR Color qPCR Master Mix |
|||
| Q411-00-100rxns | Vazyme | 100 rxns | EUR 1.09 |
ChamQ SYBR Color qPCR Master Mix |
|||
| Q411-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix |
|||
| Q411-02-500rxns | Vazyme | 500 rxns | EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix |
|||
| Q411-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 634 |
ChamQ SYBR Color qPCR Master Mix |
|||
| Q411-03-2500rxns | Vazyme | 2,500 rxns | EUR 656.51 |
2X Universal SYBR Green Fast qPCR Mix |
|||
| MBS9141672-25mL | MyBiosource | 25mL | EUR 755 |
2X Universal SYBR Green Fast qPCR Mix |
|||
| MBS9141672-5mL | MyBiosource | 5mL | EUR 255 |
2X Universal SYBR Green Fast qPCR Mix |
|||
| RK21203 | Abclonal | 1 mL | EUR 21.26 |
AceQ Universal SYBR qPCR Master Mix |
|||
| Q511-00-100rxns | Vazyme | 100 rxns | EUR 1.09 |
AceQ Universal SYBR qPCR Master Mix |
|||
| Q511-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 152.72 |
AceQ Universal SYBR qPCR Master Mix |
|||
| Q511-02-500rxns | Vazyme | 500 rxns | EUR 158.14 |
AceQ Universal SYBR qPCR Master Mix |
|||
| Q511-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 688.5 |
AceQ Universal SYBR qPCR Master Mix |
|||
| Q511-03-2500rxns | Vazyme | 2,500 rxns | EUR 712.94 |
ChamQ Universal SYBR qPCR Master Mix |
|||
| Q711-00-100rxns | Vazyme | 100 rxns | EUR 1.09 |
ChamQ Universal SYBR qPCR Master Mix |
|||
| Q711-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 152.72 |
ChamQ Universal SYBR qPCR Master Mix |
|||
| Q711-02-500rxns | Vazyme | 500 rxns | EUR 158.14 |
ChamQ Universal SYBR qPCR Master Mix |
|||
| Q711-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 688.5 |
ChamQ Universal SYBR qPCR Master Mix |
|||
| Q711-03-2500rxns | Vazyme | 2,500 rxns | EUR 712.94 |
miRNA Universal SYBR qPCR Master Mix |
|||
| MQ101-00-100rxns | Vazyme | 100 rxns | EUR 1.09 |
miRNA Universal SYBR qPCR Master Mix |
|||
| MQ101-01 | Vazyme | 125 rxn(20 μl/rxn) | EUR 38.9 |
miRNA Universal SYBR qPCR Master Mix |
|||
| MQ101-01-125rxns | Vazyme | 125 rxns | EUR 40.28 |
miRNA Universal SYBR qPCR Master Mix |
|||
| MQ101-02 | Vazyme | 500 rxn(20 μl/rxn) | EUR 145 |
miRNA Universal SYBR qPCR Master Mix |
|||
| MQ101-02-500rxns | Vazyme | 500 rxns | EUR 150.15 |
ABsolute Blue QPCR SYBR Green ROX Mix (16x 1.25ml) |
|||
| AB-4162/B | Westburg | each | Ask for price |
BrightCycle Universal SYBR Green qPCR Mix with UDG |
|||
| RK21219 | Abclonal | 5mL | EUR 29.98 |
iGreen 2X qPCR Master Mix with Low-ROX, 500Rxns-20ul |
|||
| A4183-4x125ML500Rxns | Biomatik Corporation | 4x1.25ML (500Rxns) | EUR 200.2 |
|
Description: Biotechnology |
|||
ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns |
|||
| QLMM12 | Vivantis | each | Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns |
|||
| QLMM02 | Vivantis | each | Ask for price |
